The Guardians of the Imperial Tomb are an organization founded by Itou Kashitaro once he left the Shinsengumi. Their duties appear to be similar, in that they patrol Kyoto to keep the peace, but where the Shinsengumi support the shogunate, the Guardians are imperial nationalists.

Saito Hajime and Toudou Heisuke both leave the Shinsengumi with Itou, the former as a spy.